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ARTICLE
Year : 2015  |  Volume : 4  |  Issue : 5  |  Page : 162

Infection caused by an unusual isolate of non-tuberculosis mycobacteria in Iran


1 Department of Epidemiology, Pasteur Institute of , Tehran, Iran
2 Department of Bacteriology and Virology, Alborz University of Medical Science, Karaj, Iran
3 Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, Marseille, France
4 Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Correspondence Address:
A Hashemi Shahraki
Department of Epidemiology, Pasteur Institute of Iran, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


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Aims and objectives: Sequence-based identification of non-tuberculosis mycobacteria (NTM) has become a method of choice in mycobacteriology laboratories and is accomplished by analysis of several targets, such as 16S rRNA, rpoB, hsp65, sodA, etc. A group of pigmented, sl owl-growing mycobacteria were isolated from clinical samples of four unrelated patients with which were undefined at species level by means of phenotypic tests and hsp65-PRA method. The isolates were subjected to batteries of sequencing tests for identification. Methods: From 2009 to 2014, a group of pigmented, slow-growing mycobacteria were isolated from respiratory tract specimens, soft tissue infection biopsies and abscesses of four unrelated patients across Iran. The isolates were not identified to species level by biochemical tests and hsp65-PRA. Then the isolates were subjected to identification by 16S rRNA, hsp65 and rpoB gene sequencing. Results: Based on 16S rRNA sequencing, the isolates gave a closest match (99.8%) with Mycobacterium lentiflavum ATCC 51985T of 99.4%, 99.2% and 99% with those of Mycobacterium simiae ATCC 25725T, M. triplex ATCC 700071T and Mycobacterium stomatepiae DSM 45059T, respectively. The hsp65 gene sequence (356bp) (GenBank accession: FR682913) showed the highest similarities of 98.4%, 98.1% and 97.2% with those of Mycobacterium triplex ATCC 700071T, Mycobacterium stomatepiae DSM 45059T and Mycobacterium montefiorense ATCC BAA-256T, respectively; the rpoB gene sequence (619bp) (GenBank accession: FR695853) showed the highest similarities of 95%, 94.3% and 92.7% with M. triplex ATCC 700071T, M. lentiflavum CIP 105465T and Mycobacterium sherrisii Fl-94099, respectively. Conclusions: The approaches used in the current study confirm the taxonomic status of this group of isolates as a novel Mycobacterium species. Further analysis is needed to fully characterize the isolates. The presence of unidentifiable NTM strains in the clinical setting emphasizes the need to use sequence analysis of genes for reliable identification.


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