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ARTICLE
Year : 2016  |  Volume : 5  |  Issue : 5  |  Page : 203

Molecular identification of Mycobacterium tuberculosis complex isolates from Kermanshah Province, Iran


1 Central Branch of Azad University, Iran
2 Bovine Tuberculosis Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Tehran, Iran
3 Biology Group, Central Branch of Azad University, Iran

Date of Web Publication17-Feb-2017

Correspondence Address:
Roghieh Moghaddam
Central Branch of Azad University
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.ijmyco.2016.10.026

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  Abstract 


Tuberculosis is one of the most important zoonotic diseases in the world. Rapid diagnosis of the disease and identification of species is extremely important for proper treatment of the disease as some species of the complex are resistant to the first-line of tuberculosis drugs. The aim of present study was molecular identification of Mycobacterium tuberculosis (MTB) complex isolates from Kermanshah Province, Iran, which were submitted to the Tuberculosis Reference Laboratory at Razi Vaccine and Serum Research Institute (Tehran, Iran). To identify the genus Mycobacterium, all isolates were subjected to 16S rRNA polymerase chain reaction (PCR), and PCR-IS6110 was subsequently used to confirm that the isolates belonged to MTB complex. Finally, region of difference (RD) typing was used to identify the species in the complex. The results of 16S rRNA and IS6110 PCR analysis showed the presence of 543-bp and 245-bp bands, respectively. Furthermore, 146bp, 172bp, 235bp, and 369bp at RD1, RD4, RD9, and RD12, respectively, were observed during RD typing. Thus, based on the results, all isolates were identified as MTB. It is worth mentioning that most tuberculosis cases are identified on the basis of acid-fast bacilli detection, and antibiotic therapy is immediately initiated subsequently. Moreover, it should be noted that some of these acid-fast positive cases might not be of genus Mycobacterium, and thus, the antibiotics prescribed might threaten the health of the patients. Additionally, if the identified bacilli are not within MTB complex, the drug therapy would differ. However, Mycobacterium bovis, which is a member of MTB complex and is resistant to pyrazinamide, requires exact strain identification. Based on the findings, individual isolates should be identified by RD typing methods, which could clearly discriminate the species from each other.

Keywords: IS6110, Mycobacterium tuberculosis complex, RD typing, 16S rRNA


How to cite this article:
Moghaddam R, Mosavari N, Mahalati AH. Molecular identification of Mycobacterium tuberculosis complex isolates from Kermanshah Province, Iran. Int J Mycobacteriol 2016;5, Suppl S1:203

How to cite this URL:
Moghaddam R, Mosavari N, Mahalati AH. Molecular identification of Mycobacterium tuberculosis complex isolates from Kermanshah Province, Iran. Int J Mycobacteriol [serial online] 2016 [cited 2019 Mar 21];5, Suppl S1:203. Available from: http://www.ijmyco.org/text.asp?2016/5/5/203/200426




  Conflicts of interest Top


The authors have no conflicts of interest to declare.






 

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