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ARTICLE
Year : 2016  |  Volume : 5  |  Issue : 5  |  Page : 219

Interferon-γ assay, a high-sensitivity, specific and appropriate method for detection of bovine tuberculosis in cattle


1 Bovine Tuberculosis Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran
2 ian Veterinary Organization, Tehran, Iran
3 ian Veterinary Organization, Ghazvin, Iran
4 Bovine Tuberculosis Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO); ian Veterinary Organization, Tehran; ian Veterinary Organization, Ghazvin, Iran

Date of Web Publication17-Feb-2017

Correspondence Address:
R Keshavarz
Bovine Tuberculosis Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.ijmyco.2016.10.014

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  Abstract 


Bovine tuberculosis (TB) is an important zoonotic disease that is caused by Mycobacterium bovis. Eradication efforts in developed countries have reduced the prevalence of this disease significantly. TB can be difficult to diagnose based only on the clinical signs; therefore, it is usually diagnosed in the field with the tuberculin skin test and diagnostic blood tests, including the lymphocyte proliferation assay, the interferon (IFN)-γ assay, and enzyme-linked immunosorbent assay. The aim of this study was to compare the tuberculin and IFN-γ tests. A total of 110 animals were evaluated by tuberculin skin test (TST) and IFN-γ assay; the culture was selected as a gold standard. The animals were selected randomly from 700 cattle on dairy farms, aged 3–5 years and suspected of having TB. Ten cattle were positive using the TST and nine were positive by IFN-γ assay. All nine positive samples in the IFN-γ assay were positive in culture too. The observed errors in IFN-γ assay were less due to laboratorial tools. It is suggested that all positive samples in TST are also positive by IFN-γ too.

Keywords: Bovine tuberculosis, Interferon-γ assay, Tuberculin skin test


How to cite this article:
Keshavarz R, Mosavari N, Geravand M M, Tadayon K, Pajoohi R A, Solemani K, Ameri M, Gilani G V. Interferon-γ assay, a high-sensitivity, specific and appropriate method for detection of bovine tuberculosis in cattle. Int J Mycobacteriol 2016;5, Suppl S1:219

How to cite this URL:
Keshavarz R, Mosavari N, Geravand M M, Tadayon K, Pajoohi R A, Solemani K, Ameri M, Gilani G V. Interferon-γ assay, a high-sensitivity, specific and appropriate method for detection of bovine tuberculosis in cattle. Int J Mycobacteriol [serial online] 2016 [cited 2020 Jan 23];5, Suppl S1:219. Available from: http://www.ijmyco.org/text.asp?2016/5/5/219/200437




  Conflicts of interest Top


The authors have no conflicts of interest to declare.






 

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