|Year : 2017 | Volume
| Issue : 4 | Page : 387-390
Antibiotic resistance in Mycobacterium Abscessus and Mycobacterium Fortuitum isolates from Malaysian patients
Sharmilla Devi Jayasingam, Thaw Zin, Yun Fong Ngeow
Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Selangor, Malaysia
|Date of Web Publication||17-Nov-2017|
Yun Fong Ngeow
Department of Pre-Clinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Selangor
Source of Support: None, Conflict of Interest: None
Background: Rapidly growing mycobacterial species (RGM) are increasingly being recognized as the cause of various superficial and deep infections in humans. Two of the species most frequently isolated from clinical specimens are Mycobacterium abscessus and Mycobacterium fortuitum. Both species are associated with antibiotic resistances that may complicate therapy. This paper describes the pattern of resistance to five antibiotics commonly prescribed for RGM infections, in M. abscessus and M. fortuitum isolated from Malaysian patients. Methods: The bacterial strains studied were examined with Etest strips to determine their minimum inhibitory concentrations (MICs) toward amikacin, ciprofloxacin, clarithromycin, imipenem, and linezolid. Results: Among 51 M. abscessus isolates examined by the Etest, the overall MICs of ciprofloxacin, imipenem, amikacin, clarithromycin, and linezolid showed resistance rates of 33.3%, 31.4%, 2.0%, 5.9%, and 21.6%, to the five antibiotics, respectively. M. abscessus subspecies abscessus was more resistant than M. abscessus subsp. massilience to ciprofloxacin, imipenem, and linezolid but was more susceptible to clarithromycin and amikacin. M. fortuitum isolates were significantly less resistant than M. abscessus to ciprofloxacin (3.6%) and imipenem (7.1%) but more resistant to clarithromycin (42.9%) and linezolid (39.3%). Conclusion: A suitable combination therapy for Malaysian patients would be amikacin plus clarithromycin and ciprofloxacin, to cover infections by all three M. abscessus subspecies and M. fortuitum.
Keywords: Antibiotic resistance, Mycobacterium abscessus, Mycobacterium fortuitum
|How to cite this article:|
Jayasingam SD, Zin T, Ngeow YF. Antibiotic resistance in Mycobacterium Abscessus and Mycobacterium Fortuitum isolates from Malaysian patients. Int J Mycobacteriol 2017;6:387-90
|How to cite this URL:|
Jayasingam SD, Zin T, Ngeow YF. Antibiotic resistance in Mycobacterium Abscessus and Mycobacterium Fortuitum isolates from Malaysian patients. Int J Mycobacteriol [serial online] 2017 [cited 2019 Jun 16];6:387-90. Available from: http://www.ijmyco.org/text.asp?2017/6/4/387/218630
| Introduction|| |
Rapid-growing mycobacteria (RGM) are environmental bacteria often found in water, soil, and dust. An increasing number of species have been recognized as opportunistic human pathogens and frequent isolates from clinical specimens. Among them, Mycobacterium abscessus is a formidable respiratory pathogen, frequently associated with cystic fibrosis  and causing tuberculosis-like pulmonary disease with substantial mortality. This species complex has been classified into three subspecies, M. abscessus subsp. abscessus, M. abscessus subsp. massiliense and M. abscessus subsp. bolletii, hereafter referred to here as M. abscessus, M. massiliense and M. bolletii for simplicity. The species complex, on the whole, is notorious for their resistance to multiple antibiotics, but the three subspecies differ in their geographical distribution  and susceptibility to antibiotics. To clarithromycin, a common antibiotic for the treatment of RGMs, M. massiliense is mostly susceptible, M. bolletii is most often resistant, and both M. abscessus and M. bolletii show inducible resistance.
Mycobacterium fortuitum is most often associated with skin and superficial infections acquired from community sources, such as contaminated footbaths in beauty parlors  or from contaminated water or medical devices in health-care settings. While M. abscessus has been reported to be responsible for 90% of the respiratory illness caused by RGMs, M. fortuitum is said to be responsible for 60%–80% of postsurgical and catheter-related infections caused by these bacteria. It has also been associated with serious disseminated infections involving many different organs and tissues in severely immunocompromised individuals.,, Although respiratory infections with M. fortuitum are not common, as with M. abscessus respiratory infections, M. fortuitum lung disease may be difficult to eradicate because of disease chronicity and poor response to antibiotic treatment. The antibiotic of choice for these infections is amikacin, but resistance to aminoglycosides has also been reported., In this study, strains of M. abscessus complex and M. fortuitum isolated from Malaysian patients are examined for their susceptibility to selected antibiotics to provide guidance for the empirical therapy of RGM infections in the country.
| Methods|| |
The bacterial strains studied were isolated between 2012 and 2014, from the sputum and bronchoalveolar lavage fluids of patients with clinical signs of lower respiratory tract infections. Acid-fast bacilli grown on Lowenstein-Jensen slopes within 7 days of inoculation were kept at 80°C until required for further testing. M. abscessus ATCC 19977 was used as the reference strain for the determination of minimum inhibitory concentrations (MIC).
Identification of rapidly growing mycobacterial species
The identification of M. abscessus complex and M. fortuitum was based on the DNA sequencing of the hsp 65 gene. For gene amplification, DNA was extracted from each isolate by heating a suspension of the isolate at 100°C for 15 min, followed by centrifugation at 1500 rpm for 10 min. Of the supernatant obtained, 2.5 μl was used as the DNA template in a 25 μL reaction mixture containing 6 μl of ddH2O, 12.5 μl of Master mix (Promega), and 2 μl each of forward and reverse primers. The primers used were Tb11 (5′-ACCAACGATGGTGTGTCCAT-3') and Tb12 (5′-CTTGTCGAACCGCATACCCT-3'), described by Telenti et al., 1993. The thermal cycling profile, also by Telenti, consisted of 45 cycles of denaturation at 94°C for 1 min, annealing at 60°C for 1 min, followed by extension at 72°C for 10 min. The expected product size was 439 base pairs.
Since the differentiation of M. abscessus subspecies with the hsp 65 PCR is not entirely reliable,, an additional PCR assay based on the erm (41) gene was performed to distinguish M. massiliense from the other two M. abscessus subspecies. The erm (41) gene in almost all M. massiliense is characterized by a 2 bp deletion at nucleotides 64–65 and a 274 bp deletion of nucleotides 159-432, and hence, is 276 bp shorter compared to the other two subspecies. The primers for the amplification of the erm (41) gene were erm F (5'-TGGTATCCGCTCACTGATGA-3') and erm R (5'-GCGGTGGATGTAGGAAAG-3'). The thermal cycling profile consisted of an initial denaturation at 95°C for 5 min, followed by 40 cycles of 94°C for 30 s, 55°C for 30 s, and 72°C for 60 s, and ending with a final extension at 72°C for 10 min. The expected product size was 451 bp. All amplicons obtained were purified using QIAquick PCR purification kit and were evaluated for their purity using the Thermo Scientific NanoDrop Spectrophotometer. They were then sent out for Sanger sequencing with the same primers as those used for the PCR.
Hsp 65 gene sequences were analyzed using NCBI BLASTN and hsp65BLAST (http://hsp65blast. phsa.ca/) while erm (41) gene sequences were aligned and analyzed using MEGA6 software, an integrated tool which conducts automatic and manual sequence alignments.
Determination of minimum inhibitory concentrations
All strains were examined with Etest strips (ABBiodisk, bioMe'rieux) for susceptibility to amikacin, ciprofloxacin, clarithromycin, imipenem, and linezolid, as per manufacturer's instructions. Inocula from a suspension prepared in broth to a 1 McFarland standard were plated on Mueller Hinton Blood agar. Etest strips were placed on the air-dried inoculated plates which were then incubated in an ambient air incubator at 36°C. The MICs were read after 72 h of incubation, except for clarithromycin MICs which were read on the 3rd, 7th, and 14th day of incubation, for the detection of inducible resistance. The MICs were interpreted according to the Clinical and Laboratory Standards Institute breakpoints.
| Results|| |
Subspecies identification of Mycobacterium abscessus strains
Based on the hsp 65 and erm (41) PCR results, the 51 strains of M. abscessus complex were identified as 12 strains of M. abscessus, 38 of M. massiliense, and only 1 M. bolletii. The 28 M. fortuitum strains were identified as M. fortuitum subspecies fortuitum[Table 1].
|Table 1: Summary of resistance rates in Mycobacterium abscessus complex and Mycobacterium fortuitum|
Click here to view
Antibiotic susceptibility testing
The antibiotic susceptibilities of the strains examined are summarized in [Table 1]. For all M. abscessus complex strains and M. fortuitum, amikacin seems to be the most effective antibiotic in vitro, with <4% resistance within the respective subspecies/species. Clarithromycin, likewise, showed good antimicrobial activity on the M. abscessus complex but had a rather high rate of resistance (42.9%) among M. fortuitum, with three (10.8%) of the isolates showing inducible resistance. Conversely, imipenem, which had a low rate of resistance among M. fortuitum, showed resistance rates of 21.1% to 58.3% among the M. abscessus complex. Similarly, ciprofloxacin resistance was low (3.6%) among M fortuitum but high among M. massiliense (31.6%) and M. abscessus (41.7%). Linezolid resistance increased from 18.4% in M. massiliense to 25% in M. abscessus and 39.3% in M. fortuitum. Overall, 33.3% of M. abscessus, 60.1% of M. massiliense, and 42.9% of M. fortuitum showed in vitro susceptibility to all five of the antibiotics. Of the resistant strains, none were resistant to all five antibiotics, two (5.1%; a M. massiliense and a M. fortuitum) were resistant to four antibiotics, and only seven (8.9%) were resistant to three antibiotics each. On the other hand, no antibiotic showed consistent activity on all strains. While ciprofloxacin, amikacin, and imipenem had good activity for M. fortuitum, amikacin and clarithromycin had the best activity for the M. abscessus complex.
| Discussion|| |
The goal of antimicrobial susceptibility testing is to predict whether patients treated with antibiotics are likely to be cured of their infections. For most bacteria, antibiotic susceptibilities differ substantially among strains in different geographical locations and clinical settings. Hence, for empirical therapy, local antibiotic susceptibility data are immensely helpful as a guide to the choice of antibiotics for the treatment of infections.
M. abscessus complex and M. fortuitum are not infrequently isolated from the respiratory secretions of Malaysian patients, many of whom require antibiotic treatment for their infections. We compared the antibiotic susceptibilities of our local isolates with those reported in medical literature. Although most of these isolates are single isolates and many of the infections do not satisfy the American Thoracic Society's diagnostic criteria for lung disease, the majority of culture-positive patients had a productive cough or abnormal chest X-rays and was treated with antibiotics for suspected respiratory infections.
Overall, our AST results are consistent with those reported by others , in that the greatest in vitro activity against both groups of RGM is seen in amikacin. This aminoglycoside has been the preferred treatment for M. fortuitum infections and is often used in combination with imipenem for the treatment of serious lung disease. In our series, however, the rates of imipenem resistance suggest that this combination therapy is not likely to be effective in many M. abscessus subspecies infections (21%–58% resistance) but should still be adequate for M. fortuitum infections (7.1% resistance). High rates of imipenem resistance have also been reported by others, with rates ranging from 55% to 95%.,
Fluoroquinolones have been used successfully for the treatment of RGM infections. Our results showed very good ciprofloxacin activity for M. fortuitum but relatively high rates of resistance for M. abscessus and M. massiliense. Thus, where NTM species identification is not available, fluoroquinolones might not be suitable for empirical therapy. An additional concern with the use of fluoroquinolones for respiratory infections is that these antibacterials have been associated with delayed anti-TB treatment and resistance in tuberculosis, and hence, should not be used indiscriminately in regions where the incidence of tuberculosis is high.
The macrolide clarithromycin is one of the most widely prescribed antibiotics for NTM infections, and our in vitro test results indicate that it would be a suitable antibiotic of choice for M. abscessus and M. massiliense infections. Against M. fortuitum, however, the 39% resistance we observed predicts substantial treatment failures with clarithromycin therapy. One drawback pertinent to both NTM species is the occurrence of inducible resistance in apparently clarithromycin-susceptible isolates. This is caused by the derepression of erythromycin resistance methylase (erm) genes, the erm (41) gene in M. abscessus and M. bolletii, and the erm (39) gene in M. fortuitum. These inducible resistances are not detected in routine antibiotic susceptibility tests, and hence, will not be reported to referring physicians. They are deduced when resistance emerges after the initiation of therapy. In most M. massiliense strains, the erm (41) gene is truncated and inactive. Hence, this subspecies is not affected by inducible resistance, and clarithromycin can be safely prescribed for susceptible strains.
When linezolid was first introduced for clinical use in the early 2000s, it was reported to be active against most gram-positive bacteria, including many species of RGM. However, reports of resistance among RGM soon appeared. Yang et al. found 42% resistance among clinical isolates of M. abscessus and 25% among M. fortuitum. We found similar high resistance rates of 18.4% among M. massiliense, 25% among M. abscessus, and 39.3% among M. fortuitum. The reason for this rapid emergence of resistance is still unknown, but the high prevalence of resistance indicates the need for linezolid to be used in combination with a more reliable antibiotic, in serious RGM infections.
| Conclusion|| |
In summary, our 2-year collection of RGM from respiratory secretions showed M. massiliense to be the most frequently isolated M. abscesuss subspecies and M. bolletii, the least often encountered. The rarity of M. bolletii isolation from clinical specimens is also reported from our neighboring countries, Korea  and Australia. In view of varying antibiotic susceptibilities among different RGM species and subspecies, our results reinforce the need for species and subspecies identification of RGM to ensure the choice of appropriate antibiotics for therapy. When this is not possible, combination therapy is recommended for most patients and is, especially, important in serious infections, since monotherapy may lead to the further emergence of resistance. Based on our results, a suitable combination therapy for Malaysian patients would be amikacin plus clarithromycin and ciprofloxacin, to cover infections by all three M. abscessus subspecies and M. fortuitum.
This study was funded by IPSR/RMC/UTARRF/2015-C2/N03, a research grant from Universiti Tunku Abdul Rahman, Malaysia.
Financial support and sponsorship
IPSR/RMC/UTARRF/2015-C2/N03 (Research grant from Universiti Tunku Abdul Rahman).
Conflicts of interest
There are no conflicts of interest.
| References|| |
Trovato A, Baldan R, Costa D, Simonetti TM, Cirillo DM, Tortoli E, et al.
Molecular typing of Mycobacterium abscessus
isolated from cystic fibrosis patients. Int J Mycobacteriol 2017;6:138-41.
] [Full text]
de Moura VC, da Silva MG, Gomes KM, Coelho FS, Sampaio JL, Mello FC, et al.
Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus
recovered from postsurgical infections. J Med Microbiol 2012;61:115-25.
Chua KY, Bustamante A, Jelfs P, Chen SC, Sintchenko V. Antibiotic susceptibility of diverse Mycobacterium abscessus
complex strains in New South Wales, Australia. Pathology 2015;47:678-82.
Nash KA, Brown-Elliott BA, Wallace RJ Jr. A novel gene, erm (41), confers inducible macrolide resistance to clinical isolates of Mycobacterium abscessus
but is absent from Mycobacterium chelonae
. Antimicrob Agents Chemother 2009;53:1367-76.
Redbord KP, Shearer DA, Gloster H, Younger B, Connelly BL, Kindel SE, et al.
Atypical mycobacterium furunculosis
occurring after pedicures. J Am Acad Dermatol 2006;54:520-4.
Hemmersbach-Miller M, Cárdenes-Santana MA, Conde-Martel A, Bolaños-Guerra JA, Campos-Herrero MI. Cardiac device infections due to Mycobacterium fortuitum
. Can J Infect Dis Med Microbiol 2005;16:183-5.
Brown-Elliott BA, Nash KA, Wallace RJ Jr. Antimicrobial susceptibility testing, drug resistance mechanisms, and therapy of infections with nontuberculous mycobacteria. Clin Microbiol Rev 2012;25:545-82.
Sack JB. Disseminated infection due to Mycobacterium fortuitum
in a patient with AIDS. Rev Infect Dis 1990;12:961-3.
Smith MB, Boyars MC, Woods GL. Fatal Mycobacterium fortuitum
meningitis in a patient with AIDS. Clin Infect Dis 1996;23:1327-8.
Silva TR, Petersen AL, Santos Tde A, Almeida TF, Freitas LA, Veras PS, et al.
Control of Mycobacterium fortuitum
and Mycobacterium intracellulare
infections with respect to distinct granuloma formations in livers of BALB/c mice. Mem Inst Oswaldo Cruz 2010;105:642-8.
Aínsa JA, Martin C, Gicquel B, Gomez-Lus R. Characterization of the chromosomal aminoglycoside 2'-N-acetyltransferase gene from Mycobacterium fortuitum
. Antimicrob Agents Chemother 1996;40:2350-5.
de Souza Santos DR, Lourenço MC, Coelho FS, Mello FC, Duarte RS. Resistance profile of strains of Mycobacterium fortuitum
isolated from clinical specimens. J Bras Pneumol 2016;42:299-301.
Telenti A, Marchesi F, Balz M, Bally F, Böttger EC, Bodmer T, et al.
Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 1993;31:175-8.
Macheras E, Roux AL, Ripoll F, Tardy VS, Gutierrez C, Gaillard JL, Heym B. Inaccuracy of single-target sequencing for discriminating species of the Mycobacterium abscessus
group. J Clin Microbiol 2009;47:2596-600.
Kim HY, Kim BJ, Kook Y, Yun YJ, Shin JH, Kim BJ, et al. Mycobacterium massiliense
is differentiated from Mycobacterium abscessus
and Mycobacterium bolletii
by erythromycin ribosome methyltransferase gene (erm) and clarithromycin susceptibility patterns. Microbiol Immunol 2010;54:347-53.
Maurer FP, Rüegger V, Ritter C, Bloemberg GV, Böttger EC. Acquisition of clarithromycin resistance mutations in the 23S rRNA gene of mycobacterium abscessus in the presence of inducible erm (41). J Antimicrob Chemother 2012;67:2606-11.
Clinical and Laboratory Standards Institute. Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes, Approved Standard, 2nd
ed. M24-A2, Wayne, PA: CLSI; 2011.
Park S, Kim S, Park EM, Kim H, Kwon OJ, Chang CL.In vitro
antimicrobial susceptibility of Mycobacterium abscessus
in Korea. J Korean Med Sci 2008;23:49-52.
Yang SC, Hsueh PR, Lai HC, Teng LJ, Huang LM, Chen JM, et al.
High prevalence of antimicrobial resistance in rapidly growing mycobacteria in Taiwan. Antimicrob Agents Chemother 2003;47:1958-62.
Lee MR, Sheng WH, Hung CC, Yu CJ, Lee LN, Hsueh PR, et al. Mycobacterium abscessus
complex infections in humans. Emerg Infect Dis 2015;21:1638-46.
Chihara S, Smith G, Petti CA. Carbapenem susceptibility patterns for clinical isolates of Mycobacterium abscessus
determined by the Etest method. J Clin Microbiol 2010;48:579-80.
Choi GE, Min KN, Won CJ, Jeon K, Shin SJ, Koh WJ, et al.
Activities of moxifloxacin in combination with macrolides against clinical isolates of Mycobacterium abscessus
and Mycobacterium massiliense
. Antimicrob Agents Chemother 2012;56:3549-55.
Chen TC, Lu PL, Lin CY, Lin WR, Chen YH. Fluoroquinolones are associated with delayed treatment and resistance in tuberculosis: A systematic review and meta-analysis. Int J Infect Dis 2011;15:e211-6.
Nash KA, Zhang Y, Brown-Elliott BA, Wallace RJ Jr. Molecular basis of intrinsic macrolide resistance in clinical isolates of Mycobacterium fortuitum
. J Antimicrob Chemother 2005;55:170-7.
Wallace RJ Jr., Brown-Elliott BA, Ward SC, Crist CJ, Mann LB, Wilson RW, et al.
Activities of linezolid against rapidly growing mycobacteria. Antimicrob Agents Chemother 2001;45:764-7.
Lee SH, Yoo HK, Kim SH, Koh WJ, Kim CK, Park YK, et al.
The drug resistance profile of Mycobacterium abscessus
group strains from Korea. Ann Lab Med 2014;34:31-7.