The International Journal of Mycobacteriology

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 8  |  Issue : 4  |  Page : 329--332

Validation of bedaquiline drug-susceptibility testing by BACTEC MGIT 960 system for Mycobacterium tuberculosis


Gomathi Sivaramakrishnan, Balaji Subramanyam, Michel Prem Kumar, Radhika Golla, Srikanth Prasad Tripathy, Rajesh Mondal 
 Department of Bacteriology, ICMR-National Institute for Research in Tuberculosis, Chennai, Tamil Nadu, India

Correspondence Address:
Rajesh Mondal
Department of Bacteriology, ICMR-National Institute for Research in Tuberculosis, Chennai, Tamil Nadu
India

Background: Bedaquiline (BDQ) is a new antituberculosis (TB) drug effectively used for the treatment of multidrug-resistant and extensively drug-resistant TB. However, the reports on drug-susceptibility testing (DST) for BDQ are scarce. The study aimed to validate and standardize BDQ DST by BACTEC MGIT 960 system for Mycobacterium tuberculosis. Methods: A panel of ten M. tuberculosis isolates comprising 8 BDQ sensitive and 2 BDQ resistant strains were used to test accuracy, repeatability, and reproducibility of BDQDST by MGIT 960. BDQ DST by Middlebrook 7H11 agar method using polystyrene tubes was used as a standard method to calculate the accuracy of the validation. Results: DST by MGIT for BDQ showed 100% accuracy, repeatability, and reproducibility, although variations were observed in the growth units of the “test” MGIT tubes between technologist and drug stocks while testing for reproducibility. Conclusion: BDQ DST by MGIT 960 system is accurate, repeatable, and reproducible and hence can be implemented in certified laboratories routinely performing DST by MGIT 960 system.


How to cite this article:
Sivaramakrishnan G, Subramanyam B, Kumar MP, Golla R, Tripathy SP, Mondal R. Validation of bedaquiline drug-susceptibility testing by BACTEC MGIT 960 system for Mycobacterium tuberculosis.Int J Mycobacteriol 2019;8:329-332


How to cite this URL:
Sivaramakrishnan G, Subramanyam B, Kumar MP, Golla R, Tripathy SP, Mondal R. Validation of bedaquiline drug-susceptibility testing by BACTEC MGIT 960 system for Mycobacterium tuberculosis. Int J Mycobacteriol [serial online] 2019 [cited 2020 Feb 24 ];8:329-332
Available from: http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=4;spage=329;epage=332;aulast=Sivaramakrishnan;type=0