Close
  Indian J Med Microbiol
 

Figure 2: Rv2456c is important for inhibition of cell death and survival in host cells. (A) Sequencing of the flanking region of the fails to inhibit cell death mutant 19 (FID19) transposon mutant revealed that the insertion occurred between nucleotides 305 and 306 of Rv2456c; (B) THP-1 cells were infected with H37Rv, FID19, or a complementation plasmid (FID19: pSL901) containing Rv2456c. Cells were fixed 4-days postinfection, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stained, and analyzed via flow cytometry. Data are representative of three separate experiments; (C) THP-1 cellswere infected at a multiplicity of infection of 10 with H37Rv or FID19 and plated for colony forming units at the indicated time points. Data are representative of two independent experiments; (D) bone marrow derived macrophages from C57BL/6 J mice were left untreated, treated with exogenous SIINFEKL as a positive control, or treated with either H37Rv, H37Rv expressing SIINFEKL (SIIN), or FID19 expressing SIIN. After 4 h of treatment or infection, OT-1 CD8+ T cells were added to the wells. Supernatant was collected 24-h postaddition of T cells. Secreted interferon-γ (IFNγ) was measured via enzyme-linked immunosorbent assay. Data are representative of three individual experiments; (E) spleens were harvested 3-weeks postretro-orbital intravenous infection with indicated strains and were plated for colony forming units (CFU). Data shown in (E) is a compilation of three independent experiments (2 experiments contained 4 mice/group and the third experiment contained 8 mice/group). *p < .05

Figure 2: Rv2456c is important for inhibition of cell death and survival in host cells. (A) Sequencing of the flanking region of the fails to inhibit cell death mutant 19 (FID19) transposon mutant revealed that the insertion occurred between nucleotides 305 and 306 of Rv2456c; (B) THP-1 cells were infected with H<sub>37</sub>Rv, FID19, or a complementation plasmid (FID19: pSL901) containing Rv2456c. Cells were fixed 4-days postinfection, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stained, and analyzed via flow cytometry. Data are representative of three separate experiments; (C) THP-1 cellswere infected at a multiplicity of infection of 10 with H<sub>37</sub>Rv or FID19 and plated for colony forming units at the indicated time points. Data are representative of two independent experiments; (D) bone marrow derived macrophages from C57BL/6 J mice were left untreated, treated with exogenous SIINFEKL as a positive control, or treated with either H<sub>37</sub>Rv, H<sub>37</sub>Rv expressing SIINFEKL (SIIN), or FID19 expressing SIIN. After 4 h of treatment or infection, OT-1 CD8+ T cells were added to the wells. Supernatant was collected 24-h postaddition of T cells. Secreted interferon-γ (IFNγ) was measured via enzyme-linked immunosorbent assay. Data are representative of three individual experiments; (E) spleens were harvested 3-weeks postretro-orbital intravenous infection with indicated strains and were plated for colony forming units (CFU). Data shown in (E) is a compilation of three independent experiments (2 experiments contained 4 mice/group and the third experiment contained 8 mice/group). *<i>p</i> < .05