Figure 3: Fails to inhibit cell death mutant 19-induced cell death through apoptosis. (A, B) THP-1 cellswere infected at a multiplicity of infection of 10 and supernatants were collected at indicated time points; (A) tumor necrosis factor-α (TNFα); and (B) interleukin-1β (IL-1β) concentrations were assessed via enzyme-linkedimmunosorbent assay; (C) wildtype or short hairpin RNA knockdown THP-1 cells were infected at a multiplicity of infection of 10 for 3 days with indicated strains, fixed, and terminal deoxynucleotidyl transferase dUTP nick end labeling stained (TUNEL). Data are a compilation of nine experiments; (D) THP-1 cells were infected with indicated strains, treated with dimethyl sulfoxide, 50 μM caspase 3 inhibitor, or 50 μM pan-caspase inhibitor. Cells were fixed 3-days postinfection and TUNEL stained. Data are representative of four experiments. *p < .05.