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  Indian J Med Microbiol
 

Figure 4: Nuclear factor-κB (NF-κB) activation is reduced during fails to inhibit cell death mutant 19 infection. (A) Nuclear factor κB transcription factors p50 and p65 in nuclear extracts of THP-1 cells infected for 2 days with indicated strains were measured via enzyme-linked immunosorbent assay; (B) THP-1 cells were infected with indicated strains and treated with dimethyl sulfoxide or BAY11-7082 for 3- or 4-days postinfection, after which cells were fixed, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stained, and analyzed by flow cytometry. Data are presented as terminal deoxynucleotidyl transferase dUTP nick end labeling-positive BAY11-7082-treated cells over dimethyl sulfoxide control cells and is representative of two experiments; (C) THP-1 cells were infected for the duration of time indicated and protein lysates were acquired and probed for indicated protein; (D, E) densitometry calculations of (C) are shown as a ratio over the β-actin loading control and are a compilation of three separate experiments. *p < .05.

Figure 4: Nuclear factor-κB (NF-κB) activation is reduced during fails to inhibit cell death mutant 19 infection. (A) Nuclear factor κB transcription factors p50 and p65 in nuclear extracts of THP-1 cells infected for 2 days with indicated strains were measured via enzyme-linked immunosorbent assay; (B) THP-1 cells were infected with indicated strains and treated with dimethyl sulfoxide or BAY11-7082 for 3- or 4-days postinfection, after which cells were fixed, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) stained, and analyzed by flow cytometry. Data are presented as terminal deoxynucleotidyl transferase dUTP nick end labeling-positive BAY11-7082-treated cells over dimethyl sulfoxide control cells and is representative of two experiments; (C) THP-1 cells were infected for the duration of time indicated and protein lysates were acquired and probed for indicated protein; (D, E) densitometry calculations of (C) are shown as a ratio over the β-actin loading control and are a compilation of three separate experiments. *<i>p</i> < .05.