Figure 5: Fails to inhibit cell death mutant 19 has enhanced immunogenicity when compared with parental strain H37Rv. (A) C57BL/6 J mice were infected with indicated strain for 3 weeks. Splenocytes were then isolated, stimulated for 24 h with either a CD8+-specific antigen (Tb10.4) or a CD4+-specific antigen (Ag85) for enzyme-linked immunoSpot analysis. Data are representative of three experiments (2 experiments contained 4 mice/group and the third experiment contained 8 mice/group); (B) splenocytes were isolated, stimulated for 24 h with either a CD8+-specific antigen (Tb10.4) or a CD4+-specific antigen (Ag85). After the incubation, the cells were stained for CD4+ and CD8+ surface markers and interleukin-2, interferon-γ (IFNγ), and tumor necrosis factor-α for intracellular cytokine staining analysis. The data show a representative figure of a standard experiment with four mice/group. Note: CFU = colony forming units; SFU = spot forming units. *p < .05.