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  Indian J Med Microbiol
 

Figure 1: Representative thin-layer chromatography from lymph node tuberculosis and pulmonary tuberculosis isolates. Lane 1: Control, Lane 2: Surface stress (sodium dodecyl sulfate 0.05%). Equal quantities (5 μl) of extracted mycolic acids from each clinical isolate were spotted by capillary on silica gel-coated plates. Thin-layer chromatography was developed three times in n-Hexane and diethyl ether (85:15, v/v). Thin-layer chromatography (a) Mycolic acid of the standard strain Mycobacterium tuberculosis H37Rv; thin-layer chromatography (b and c) Mycolic acids extracted from lymph node tuberculosis; thin-layer chromatography (d and e) Mycolic acids extracted from pulmonary tuberculosis

Figure 1: Representative thin-layer chromatography from lymph node tuberculosis and pulmonary tuberculosis isolates. Lane 1: Control, Lane 2: Surface stress (sodium dodecyl sulfate 0.05%). Equal quantities (5 μl) of extracted mycolic acids from each clinical isolate were spotted by capillary on silica gel-coated plates. Thin-layer chromatography was developed three times in n-Hexane and diethyl ether (85:15, v/v). Thin-layer chromatography (a) Mycolic acid of the standard strain <i>Mycobacterium tuberculosis</i> H37Rv; thin-layer chromatography (b and c) Mycolic acids extracted from lymph node tuberculosis; thin-layer chromatography (d and e) Mycolic acids extracted from pulmonary tuberculosis