|Year : 2016 | Volume
| Issue : 2 | Page : 192-196
Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacterium smegmatis
Jean Bosco Jouda1, Isabelle Kamga Mawabo2, Augustin Notedji3, Céline Djama Mbazoa4, Jean Nkenfou3, Jean Wandji4, Céline Nguefeu Nkenfou5
1 Molecular Biology Center; Department of Organic Chemistry, University of Yaoundé I, Yaoundé, Cameroon
2 Molecular Biology Center, Yaoundé; Department of Biochemistry, University of Dschang, Dschang, Cameroon
3 Molecular Biology Center, Yaoundé, Cameroon
4 Department of Organic Chemistry, University of Yaoundé I, Yaoundé, Cameroon
5 Molecular Biology Center; Higher Teacher Training College, University of Yaoundé I; Centre International de Référence Chantal Biya, Yaoundé, Cameroon
|Date of Web Publication||9-Feb-2017|
Céline Nguefeu Nkenfou
Molecular Biology Center, P.O. Box 14495, Yaoundé
Source of Support: None, Conflict of Interest: None
Objective/background: According to estimates by the World Health Organization, there were 9.6 million new tuberculosis (TB) cases in 2014: 5.4 million among men, 3.2 million among women, and 1.0 million among children. There were also 1.5 million TB deaths. Although there are potent anti-TB molecules, the misuse of these drugs in addition to inconsistent or partial treatment have led to the development of multidrug-resistant TB and extensively drug-resistant TB. It is established that plants harbor microorganisms, collectively known as endophytes, which also produce metabolites. Exploring the as-yet untapped natural products from the endophytes increases the chances of finding novel and active compounds. The present study was aimed to investigate the antimycobacterial activity of the crude extract and compounds isolated from Penicillium sp. endophyte associated with Garcinia nobilis against Mycobacterium smegmatis.
Methods: Liquid culture obtained from the fermentation of Penicillium sp. was extracted using ethylacetate and the liquid chromatography–mass spectrometry monitored fractionation of crude extracts yielded six compounds. Their structures were elucidated with spectroscopic analyses including two-dimensional nuclear magnetic resonance, high resolution mass spectrometry by dereplication using Antibase, and by comparison to literature data. All compounds and the crude extract from the liquid medium were evaluated for their antimycobacterial activity against M. smegmatis.
Results: In this study, the activity of penialidins A–C (1–3), citromycetin (4), p-hydroxy phenyl glyoxalaldoxime (5), and Brefeldin A (6) were tested against nonpathogenic M. smegmatis. Penialidin C was the most active compound with a minimum inhibitory concentration of 15.6μg/mL.
Conclusion: Isolated compounds from Penicillium sp. harbored in G. nobilis exhibited promising antimycobacterial activity against M. smegmatis thus supporting the immensity of the potential of antimycobacterial drug discovery from endophytes from medicinal plants. Penialidin C could further be investigated for antimycobacterial drug development.
Keywords: Antimycobacterial, Endophyte, Garcinia nobilis, Penicillium, Polyketides
|How to cite this article:|
Jouda JB, Mawabo IK, Notedji A, Mbazoa CD, Nkenfou J, Wandji J, Nkenfou CN. Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacterium smegmatis. Int J Mycobacteriol 2016;5:192-6
|How to cite this URL:|
Jouda JB, Mawabo IK, Notedji A, Mbazoa CD, Nkenfou J, Wandji J, Nkenfou CN. Anti-mycobacterial activity of polyketides from Penicillium sp. endophyte isolated from Garcinia nobilis against Mycobacterium smegmatis. Int J Mycobacteriol [serial online] 2016 [cited 2021 May 11];5:192-6. Available from: https://www.ijmyco.org/text.asp?2016/5/2/192/199929
| Introduction|| |
Tuberculosis (TB) is currently a major public health problem due to the advent of multidrug-resistant (MDR) forms of bacilli as well as human immunodeficiency virus epidemics. Global efforts are underway to eradicate TB using new drugs with new modes of action, higher activity, and fewer side effects. For this reason, unexplored new sources and previously explored sources were examined and around 353 antimycobacterial compounds  have been previously reported. To develop drugs from these new sources, additional work is required for preclinical and clinical evaluation. In 2014, there were an estimated 9.6million new TB cases: 5.4 million among men, 3.2 million among women, and 1.0 million among children. Also, there were 1.5 million TB deaths ,. A potent antitubercular drug rifampicin (RMP), which was introduced 50years ago, is still currently being used for treatment in combination with isoniazid (INH), ethambutol (EMB), and pyrazinamide (PZA) as a multidrug regimen for a period of at least 6months. The misuse of these drugs in addition to inconsistent or partial treatment has led to the development of MDR-TB and extensively drug-resistant TB. The resistant strains coupled with drug hepatotoxicity and lengthy therapy paved the way for a TB therapeutic crisis ,,,. This situation presently acts as a serious challenge to the health care system. Thus, it becomes necessary to prioritize the search for new antimycobacterial agents worldwide ,. As part of our research activities in discovering potent anti-TB compounds  and based on our previous investigation on the antibacterial activity of some compounds , we further our investigation with the determination of the activities of metabolites produced by endophytes on mycobacteria, subject of this report.
| Materials and methods|| |
General experimental procedures
The high resolution mass spectra were obtained with an LTQ-Orbitrap Spectrometer (Thermo Fisher, Waltham, MA, USA) equipped with a HESI-II source. The spectrometer was operated in positive mode (1 spectrum/s; mass range: 100–1000) with nominal mass resolving power of 60,000 at m/z 400 with a scan rate of 1Hz) with automatic gain control to provide high-accuracy mass measurements within 2ppm deviation using an internal standard, bis (2-ethylhexyl) phthalate: m/z = 391.28428. The spectrometer was attached with an Agilent (Santa Clara, CA, USA) 1200 High Performance Liquid Chromatography (HPLC) system consisting of a LC-pump, photodiode array detector (λ = 260nm), auto sampler (injection volume 5μL), and column oven (30°C). The following parameters were used for experiments: spray voltage 5kV, capillary temperature 260°C, and tube lens 70V. Nitrogen was used as a sheath gas (50 arbitrary units) and auxiliary gas (5 arbitrary units). Helium served as the collision gas. The high resolution mass spectra of compounds was performed on a preparative HPLC using a Nucleodur C18 gravity column (50 mm×2 mm, 1.8-μm particle size) with a H2O (+0.1% HCOOH; A)/acetonitrile (+0.1% HCOOH; B) gradient (flow rate 350μL/min). Samples were analyzed using a gradient program as follows: 95% A isocratic for 10min, linear gradient to 100% B over 14min, after 100% B isocratic for 4min, the system returned to its initial condition (95% A) within 0.5min, and was equilibrated for 4.5min (flow rate 350μL/min). The purification was carried out using a preparative HPLC run for 20min on a Gilson apparatus with UV detection at 220nm using a Nucleodur C18 Isis column (Macherey–Nagel, Düren, Germany), 5μm (250 mm×16 mm) with a H2O (A)/CH3OH (B) gradient (flow rate 4mL/min). Samples were separated using a gradient program as follows: 60% A isocratic for 2min, linear gradient to 100% B over 18min, after 100% B isocratic for 5min, the system returned to its initial condition (60% A) within 0.5min, and was equilibrated for 4.5min. Silica gel (Merck, Kenilworth, NJ, USA [0.063–0.200 mm]) was used for column chromatography. The nuclear magnetic resonance (NMR) spectra were recorded on a Bruker DRX-500MHz, Bruker BioSpin, 34 RUE DE L INDUSTRIE. 67160, Wissembourg, France, spectrometer.
Sampling of Garcinia nobilis
Leaves of G. nobilis were collected in Mount Etinde in the Southwest Region of Cameroon. The plant material was identified by Mr. Victor Nana, botanist at the Cameroon National Herbarium (Yaoundé, Cameroon) under a voucher specimen (50779/HNC/Cam/Mt Zamangoué).
Isolation and identification of the endophytic fungus
The healthy leaf was first cleaned by washing it several times under running tap water and then cut into small slices, followed by successive surface sterilization in 70% ethanol and NaOCl (6–14% active chlorine) for 2min and finally with sterile distilled water for two to three times. Plant material was then dried in between the folds of sterile filter papers and deposited on a petri dish containing potato dextrose agar medium (20g of potato, 20g of dextrose, and 15g of agar in 1L of H2O [supplemented with 100mg/L of chloramphenicol to suppress bacterial growth]) and incubated at 25°C until the growth of endophytic fungi was discerned. Hyphal tips originating from plant segments were transferred to potato dextrose agar. Each fungal isolate was checked for purity and transferred to the new medium using the hyphal tip method. One of the isolates, CAM64, was selected for further studies based on its morphotype and the liquid chromatography–mass spectrometry profile of its crude extract from small scale fermentation. The fungus was identified as Penicillium sp., as previously described .
Fermentation, extraction, and isolation of secondary metabolites
Fermentation and extraction from potato dextrose broth medium were described during our previous investigation of this fungus. Compounds tested herein, penialidins (A–C) and citromycetin (1–4; [Figure 1]) were obtained as we previously reported for their isolation and characterization from potato dextrose broth medium of the investigated fungus .
|Figure 1: Structures of penialidin A–C (1–3), citromycetin (4), p-hydroxy-phenyl-glyoxalaldoxime (5), and Brefeldin A (6).|
Click here to view
The activities were screened using the Alamar Blue method as previously described. A modified twofold serial dilution of extract/compound in 96-well microtiter plates was used to detect antimycobacterial activity ,. Isolated compounds were serially diluted (100μL) with albumin dextrose saline supplemented with Middlebrook 7H9 broth in the wells of a microtiter plate before mycobacterial culture (100μL) was added to each well. The combined anti-TB drugs RMP 150mg, INH 75mg, EMB 275mg, and pyrazinamide 400mg was used as a positive control, and media controls were included. Various dilutions were tested in triplicate and the entire experiment was repeated.
The microplates were sealed with parafilm and placed in an environmental shaker; this was incubated at 37°C for 48h. Minimal inhibitory concentration (MIC) values were detected using a resazurin indicator at 0.2mg/mL. The color reaction after the addition of resazurin generally occurred after a 2-h incubation period. MIC values were read as those concentrations where a pronounced change of color formation was noticed (from blue to orange).
| Results|| |
From the fermentation of this fungus, the crude extract (3.2g) was first partitioned with cyclohexane to remove fatty material. The resulted polar fraction (1.8g) was then subjected to column chromatography using Sephadex LH-20 to yield four main fractions (Fr1–Fr4) after thin layer chromatography monitoring. Fractions Fr2 (96mg) and Fr3 (105mg) were purified by preparative HPLC eluting with a gradient of MeOH−H2O+ 0.1%TFA to yield p-hydroxy-phenyl glyoxalaldoxime (5) C8H8O3N (4.6mg, tR = 6.73min, HRESIMS at 166.05022 [M+H]+: (calculated 166.05042), and Brefeldin A (6) C16H24O4 (3.5mg, tR = 12.06min, HRESIMS at 281.17957 [M+H]+: (calculated 281.17528). Their structures ([Figure 1]) were determined from their high resolution mass spectrometry combined to 1H and 13C NMR, by introducing these data into Antibase  and finally confirmed by two-dimensional NMR spectra. The chemical shifts were in agreement with those reported in the literature .
1H NMR and 13C NMR data of compounds 5 and 6
p-Hydroxy-phenyl glyoxalaldoxime (5): 1H NMR (500MHz, CD3OD, δ [ppm], J [Hz]): δ 8.05 (1H, d, 8.8, H-2/2′), 6.87 (2H, m, H-3/3′), 8.04 (1H, s, H-6), and 13C NMR (500MHz, CD3OD,δ [ppm]): δ 138.9 (C-1), 147.6 (C-2/2′), 115.1 (C-3/3′), 163.2 (C-4), 187.7 (C-5), 105.0 (C-6).
Brefeldin A (6): 1H NMR (500MHz, CD3OD, δ [ppm], J [Hz]): δ 7.47 (1H, dd, 15.6, 3.1, H-2), 5.85 (1H, dd, 15.6, 1.9, H-3), 4.06 (1H, dt, 4.6, 2.5, H-4), 1.86 (1H, m, H-5), 2.04 (1H, m, H-6a), 1.83 (1H, m, H-6b), 4.24 (1H, m, H-7), 2.16 (1H, m, H-8a), 1.47 (1H, m, H-8b), 2.40 (1H, m, H-9), 5.29 (1H, m, H-10), 5.76 (1H, m, H-11), 2.04 (1H, m, H-12a), 1.88 (1H, m, H-12b), 0.95 (2H, m, H-13), 1.79 (1H, m, H-14a), 1.61 (1H, m, H-14b), 4.82 (1H, d, 1.1, H-15), 1.27 (3H, d, 6.3, H-16), and 13C NMR (500MHz, CD3OD,δ [ppm]): δ 167.3 (C-1), 154.1 (C-2), 116.8 (C-3), 75.2 (C-4), 52.1 (C-5), 40.5 (C-6), 71.9 (C-7), 43.1 (C-8), 44.0 (C-9), 137.0 (C-10), 130.9 (C-11), 31.9 (C-12), 10.5 (C-13), 34.0 (C-14), 72.1 (C-15), and 20.0 (C-16).
All compounds and the crude extract from the liquid medium were tested for their antimycobacterial activities and compared with the positive control. Results are provided in [Table 1]. Penialidin C was the most active compound with a MIC of 15.6μg/mL, followed by citromycetin with a MIC of 31.2μg/mL and RMP/INH/EMB/PZA used as a reference drug in the assay having a MIC value of 0.62μg/mL.
|Table 1: Antimycobacterial activity of compounds 1–6 compared with a standard reference.|
Click here to view
| Discussion|| |
Our study showed that penialidin C, isolated from the endophyte Penicillium sp. harbored by G. nobilis, has antimycobacterial activity.
G. nobilis is found in the Southwest Region of Cameroon and is known to be a rich source of bioactive xanthones ,. Similar to the small peptides, polyketides constitute a large proportion of industrial antibiotics. More than 300 novel antitubercular agents were identified and characterized from biological sources between 2003 and 2005, while there were a further 450 novel entities identified from 2006 to 2009. Furthermore, there have been 28 novel compounds isolated from microbial sources between 2008 and 2012. Of these, 11 were polyketides or polyketide derivatives .
WHO declared TB as global emergency because of the increase of TB cases with the advent of human immunodeficiency virus/AIDS infection and the emergence of MDR and extensively drug-resistant TB strains . No new antimycobacterial has been introduced in the past 30years; hence, there is an urgent need to develop novel, safe, effective, and affordable antitubercular agents. There has been a renewed interest in microbes as a source of novel bioactive compounds in the past decade. Thus, endophytes have been investigated for various pharmacological effects including antimycobacterial activity ,. Medicinal plants are recognized as repository for fungal endophytes with metabolites containing novel molecular structures and biologically active compounds against various human pathogenic diseases for potential use in modern medicine . A number of endophytic fungi from some Garcinia species have been found to produce new antitubercular secondary metabolites ,.
The observed activities of these compounds support the immensity of the potential of antimycobacterial drug discovery from endophytes from medicinal plants. Penialidin C was identified in our study as having antimycobacterial activity and could be further investigated for its development as an anti-TB drug.
| Conflicts of interest|| |
We declare that we have no conflicts of interest.
| Acknowledgments|| |
This work was supported by grants of the German Academic Exchange Service (DAAD), grant A/12/90548 to Jean-Bosco Jouda for his PhD studies.
| References|| |
B.R. Copp, A.N. Pearce, Natural product growth inhibitors of Mycobacterium tuberculosis, Nat. Prod. Rep. 24 (2007) 278–297.
C. Dye, B.G. Williams, The population dynamics and control of tuberculosis, Science 328 (2010) 856–861.
World Health Organization, Multidrug and Extensively Drug Resistant TB (M/XDR-TB): 2010 Global Report on Surveillance and Response, WHO, Geneva, 2010.
M. Amin, S. Segatoleslami, M. Hashemzadeh, Antimycobacterial activity of partial purified extract of Allium ascalonicum, J. Microbiol. 2 (2009) 144–147.
A. Mann, J.O. Amupitan, A.O. Oyewale, et al, An ethnobotanical survey of indigenous flora for treating tuberculosis and other respiratory diseases in Niger State, Nigeria, J. Phytomed. Ther. 12 (2007) 1–12.
C. Dye, S. Scheele, P. Dolin, et al, Consensus statement. Global burden of tuberculosis: estimated incidence, prevalence, and mortality by country, WHO Global Surveillance and Monitoring Project, JAMA 282 (1999) 677–686.
A. Speck, M.T. Scotti, V. de Paulo, Current pharmaceutical design of antituberculosis drugs: future perspectives, Curr. Pharm. Des. 16 (2010) 2656–2665.
A. Jiménez, M. Meckes, R. Ramírez, et al, Activity against multidrug-resistant Mycobacterium tuberculosis in Mexican plants used to treat respiratory diseases, Phytother. Res. 17 (2003) 903–908.
C.N. Nkenfou, I.K. Mawabo, A. Notedji, et al, In vitro antimycobacterial activity of six Cameroonian medicinal plants using microplate Alamar Blue assay, Int. J. Mycobacteriol. 4 (2015) 306–311.
J.B. Jouda, S. Kusari, M. Lamshö ft, et al, Penialidins A-C with strong antibacterial activities from Penicillium
sp., an endophytic fungus harboring leaves of Garcinia nobilis, Fitoterapia 98 (2014) 209–214.
V. Seidel, P.W. Taylor, In vitro activity of extracts and constituents of Pelagonium against rapidly growing mycobacteria, Int. J. Antimicrob. Agent 23 (2004) 613–619.
L.J. McGaw, N. Lall, T.M. Hlokwe, et al, Purified compounds and extracts from Euclea species with antimycobacterial activity against Mycobacterium bovis and fast-growing mycobacteria, Biol. Pharm. Bull. 31 (2008) 1429–1433.
H. Laatsch, AntiBase, A Data Base for Rapid Dereplication and Structure Determination of Microbial Natural Products,Wiley VCH, Weinheim, Germany, 2013.
V. Betina, Biological effects of the antibiotic brefeldin A (Decumbin, Cyanein, Ascotoxin, Synergisidin): a retrospective, Folia Microbiol. 37 (1992) 3–11.
H. Fouotsa, J.N.S. Tatsimo, D.C. Neumann, et al, A new xanthone derivative from twigs of Garcinia nobilis, Nat. Prod. Res. 28 (2014) 1030–1036.
H. Fouotsa, A.M. Lannang, D.C. Mbazoa, et al, Xanthones inhibitors of a-glucosidase and glycation from Garcinia nobilis, Phytochem. Lett. 5 (2012) 236–239.
A. Alvin, K.I. Miller, B.A. Neilan, Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds, Microbiol. Res. 169 (2014) 483– 495.
T.F. Brewer, S.J. Heymann, To control and beyond: moving towards eliminating the global tuberculosis threat, J. Epidemiol. Community Health 58 (2004) 822–825.
V. Sonaimuthu, S. Parihar, J.P. Thakur, et al, Tenuazonic acid: a promising antitubercular principle from Alternaria alternate, Microbiol. Res. 2 (2011) 63–65.
M.E. Bungihan, M.A. Tan, M. Kitajima, et al, Bioactive metabolites of Diaporthe sp. P133, an endophytic fungus isolated from Pandanus amaryllifolius, J. Nat. Med. 65 (2011) 606–609.
K. Sanjana, G. Suruchi, A. Maroof, et al, Endophytic fungi from medicinal plants: a treasure hunt for bioactive metabolites, Phytochem. Rev. 11 (2012) 487–505.
M. Isaka, A. Jaturapat, K. Rukseree, et al, Phomoxanthones A and B, novel xanthone dimers from the endophytic fungus Phomopsis species, J. Nat. Prod. 64 (2001) 1015–1018.
V. Rukachaisirikul, U. Sommart, S. Phongpaichit, et al, Metabolites from the endophytic fungus Phomopsis sp. PSUD15, Phytochemistry 69 (2008) 783–787.
|This article has been cited by|
||Endophytic Penicillium species and their agricultural, biotechnological, and pharmaceutical applications
| ||Rufin Marie Kouipou Toghueo,Fabrice Fekam Boyom |
| ||3 Biotech. 2020; 10(3) |
|[Pubmed] | [DOI]|
||Caspicaiene: a new kaurene diterpene with anti-tubercular activity from an Aspergillus endophytic isolate in Gleditsia caspia desf
| ||Ashaimaa Y. Moussa,Hagar A. Sobhy,Omayma A. Eldahshan,Abdel Nasser B. Singab |
| ||Natural Product Research. 2020; : 1 |
|[Pubmed] | [DOI]|
||Computer-Assisted Drug Virtual Screening Based on the Natural Product Databases
| ||Baoyu Yang,Jing Mao,Bing Gao,Xiuli Lu |
| ||Current Pharmaceutical Biotechnology. 2019; 20(4): 293 |
|[Pubmed] | [DOI]|
||Two metabolites isolated from endophytic fungus Coniochaeta sp. F-8 in Ageratina adenophora exhibit antioxidative activity and cytotoxicity
| ||Jie Fu,Liwen Hu,Zhen Shi,Wei Sun,Dong Yue,Ya Wang,Xiaoping Ma,Zhihua Ren,Zhicai Zuo,Guangneng Peng,Zhijun Zhong,Junliang Deng,Suizhong Cao,Yanchun Hu |
| ||Natural Product Research. 2019; : 1 |
|[Pubmed] | [DOI]|
||Flupyranochromene, a novel inhibitor of influenza virus cap-dependent endonuclease, from Penicillium sp. f28743
| ||Manabu Yamasaki,Masayuki Igarashi,Ryuichi Sawa,Chisato Nosaka,Maya Umekita,Masaki Hatano,Tomoyuki Kimura,Kiyoko Iijima,Naoki Takizawa,Taira Kato,Kiyohisa Mizumoto,Akio Nomoto |
| ||The Journal of Antibiotics. 2019; |
|[Pubmed] | [DOI]|
||In-vitro Antimycobacterial Activities of Endophytic Bacteria Associated with Medicinal Plant of Manipur
| ||Tamreihao K |
| ||Journal of Bacteriology & Mycology: Open Access. 2017; 4(4) |
|[Pubmed] | [DOI]|
||Peniproline A, a new 1-phenylamino-2-pyrrolidone metabolite from the endophytic fungus Penicillium decumbens CP-4
| ||Xiang Wang,Jun Li,Shui Yu,Li Ye,Meiqing Feng,Jiyang Li |
| ||Natural Product Research. 2017; : 1 |
|[Pubmed] | [DOI]|
||Endophytes as sources of antibiotics
| ||Elena Martinez-Klimova,Karol Rodríguez-Peña,Sergio Sánchez |
| ||Biochemical Pharmacology. 2017; 134: 1 |
|[Pubmed] | [DOI]|
||Proteomic profile of Mycobacterium tuberculosis after eupomatenoid-5 induction reveals potential drug targets
| ||Luciana D Ghiraldi-Lopes,Paula AZ Campanerut-Sá,Jean E Meneguello,Flávio AV Seixas,Mariana A Lopes-Ortiz,Regiane BL Scodro,Claudia TA Pires,Rosi Z da Silva,Vera LD Siqueira,Celso V Nakamura,Rosilene F Cardoso |
| ||Future Microbiology. 2017; |
|[Pubmed] | [DOI]|