• Users Online: 365
  • Home
  • Print this page
  • Email this page


 
 Table of Contents  
ARTICLE
Year : 2016  |  Volume : 5  |  Issue : 5  |  Page : 159-160

Molecular genetics of Mycobacterium tuberculosis resistant to aminoglycosides and cyclic peptide testing by MTBDRsl in Armenia


1 Ministry of Health of National Tuberculosis Control Program SNPO, Abovyan, Armenia
2 Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Date of Web Publication17-Feb-2017

Correspondence Address:
Hasmik Margaryan
Ministry of Health of National Tuberculosis Control Program SNPO, Abovyan, 10 Arzni Avenue
Armenia
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.ijmyco.2016.07.010

Rights and Permissions
  Abstract 


Objective/background: The GenoType MTBDRsl test rapidly detects resistance to ethambutol, fluoroquinolones, second-line aminoglycosides (amikacin [AMK] and kanamycin [KAN]), and cyclic peptides (capreomycin [CAP]) in Mycobacterium tuberculosis. According to data from Global Drug Resistance Surveillance Report (2007), Armenia is counted as a high-burden country for multidrug-resistant tuberculosis (MDR-TB). The estimated burden of MDR-TB in 2012 was 9.4 (7–12) and 43 (38–49) among retreatment TB cases. A total of 92 laboratory confirmed cases were reported to the World Health Organization (57 new and 35 previously treated) out of 511 cases tested for MDR-TB.
Methods: A set of 77 drug-resistant TB isolates during 2011 and 2012 period, being either acid-fast bacterium positive or negative but culture-positive resistant to isoniazid, rifampin, or both according to the GenoType MTBDR plus assay, were consecutively tested using GenoType MTBDRsl. rrs gene analysis and the results from GenoType MTBDRsl were compared with phenotypic drug resistance testing. The DNA preparation method was performed as recommended by the manufacturer (Genotype MTBDR plus version 1.0 and Genotype MTBDRsl version 2.0 Hain Lifescience Nehren, Germany).
Results: Aminoglycosides are key drugs for the treatment of MDR-TB. A total of 77 drug-resistant TB and four extensively drug-resistant M. tuberculosis isolates from Armenian TB patients were analyzed to characterize mutations within rrs and to compare with phenotypic drug resistance testing. Simultaneously, the following were identified: 65 (84.41%) rrs wild type (WT), 1 (1.3%) rrs WT MUT1 and MUT2 (WT; A1401G and G1484T), 1 (1.3%) rrs WT1, MUT1 (A1401G), 9 (11.7%) rrs WT1, MUT1 (A1401G), and 1 (1.3%) rrs WT1, MUT1. Mutation at position 1401 in rrs leads to resistance to KAN (7/77 = 9%), AMK (9/77 = 11.68%), and CAP (5/77 = 6.49%). Eleven (14.28%) streptomycin-resistant strains had a rrs mutation.
Conclusion: Isolates with rrs structural gene mutations were cross-resistant to streptomycin, KAN, CAP, and AMK. Detection of the A1401G mutation appeared to be 100% specific for the detection of resistance to KAN and AMK. Being the first assessment, these data establish the presence of phenotypic drug-resistant and extensively drug-resistant strains using molecular profiling and are helpful in understanding aminoglycoside resistance on a molecular level.

Keywords: Aminoglycoside resistance, Mutation, XDR


How to cite this article:
Margaryan H, Farnia P, Hayrapetyan A, Mirzoyan A. Molecular genetics of Mycobacterium tuberculosis resistant to aminoglycosides and cyclic peptide testing by MTBDRsl in Armenia. Int J Mycobacteriol 2016;5, Suppl S1:159-60

How to cite this URL:
Margaryan H, Farnia P, Hayrapetyan A, Mirzoyan A. Molecular genetics of Mycobacterium tuberculosis resistant to aminoglycosides and cyclic peptide testing by MTBDRsl in Armenia. Int J Mycobacteriol [serial online] 2016 [cited 2021 May 11];5, Suppl S1:159-60. Available from: https://www.ijmyco.org/text.asp?2016/5/5/159/200396




  Conflict of interest Top


There is no conflict of interest to declare.





This article has been cited by
1 Molecular Diagnostics for Mycobacterium tuberculosis Infection
Kristen V. Dicks,Jason E. Stout
Annual Review of Medicine. 2019; 70(1): 77
[Pubmed] | [DOI]



 

Top
 
 
  Search
 
Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

 
  In this article
Abstract
Conflict of interest

 Article Access Statistics
    Viewed846    
    Printed22    
    Emailed0    
    PDF Downloaded68    
    Comments [Add]    
    Cited by others 1    

Recommend this journal