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ARTICLE
Year : 2016  |  Volume : 5  |  Issue : 5  |  Page : 74-75

Comparison of two molecular methods and an automated liquid culture system for the early detection of Mycobacterium tuberculosis from both pulmonary and extrapulmonary specimens in Kuwait


1 Department of Microbiology, Faculty of Medicine, University; National TB Reference Laboratory, Kuwait
2 National TB Reference Laboratory, Kuwait
3 Department of Microbiology, Faculty of Medicine, University, Kuwait

Date of Web Publication17-Feb-2017

Correspondence Address:
E M Mokaddas
Department of Microbiology, Faculty of Medicine, Kuwait University, PO Box 11238, Dasma 35153
Kuwait
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Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.ijmyco.2016.09.004

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  Abstract 


Objective/background: Molecular methods for the detection of Mycobacterium tuberculosis (MTB) are widely used for the early diagnosis of tuberculosis (TB). The objectives of this study were to evaluate the performance of two molecular techniques for the detection of MTB for both pulmonary and extrapulmonary specimens in comparison with conventional culture techniques in Kuwait and to correlate the results with clinical and histopathological diagnosis.
Methods: A total of 9,594 consecutive pulmonary (n = 5,597) and extrapulmonary (n = 3,997) specimens received by the Kuwait National TB Reference Laboratory from November 2011 to December 2015 were processed for direct smear microscopy for acid-fast bacilli using a Zhiel-Neelsen stain, Xpert MTB/RIF assay, ProbTec polymerase chain reaction (PCR), and growth in fully automated MGIT 960 system tubes. The clinical and histopathological diagnoses were correlated with the results.
Results: For pulmonary specimens, the overall sensitivity and specificity of both molecular methods together were 90.7% and 99.7%, respectively. The sensitivity and specificity of the Xpert MTB/RIF assay were 90.1% and 99.4%, respectively. The sensitivity and specificity of ProbTec PCR were 86% and 99.6%, respectively. For extrapulmonary specimens, the overall sensitivity and specificity of both molecular methods together were 87% and 97.8%, respectively. The sensitivity and specificity of Xpert MTB/RIF assay were 89.5% and 97.2%, respectively. The sensitivity and specificity of ProbTec PCR were 73% and 97.8%, respectively. When clinical and histopathological correlations were made with the assumed false-positive results from both molecular methods, the corrected sensitivity and specificity for pulmonary specimens were 91% and 99.9%, respectively, and 89.6% and 99.8% for extrapulmonary specimens, respectively.
Conclusion: The performance of the Xpert MTB/RIF assay was superior to ProbTec PCR for the early detection of MTB from both pulmonary and extrapulmonary specimens.

Keywords: Mycobacterium tuberculosis, Pulmonary, Extrapulmonary, GeneXpert TB/REF, ProbTec


How to cite this article:
Mokaddas E M, Saadaldeen H, Ahmad S. Comparison of two molecular methods and an automated liquid culture system for the early detection of Mycobacterium tuberculosis from both pulmonary and extrapulmonary specimens in Kuwait. Int J Mycobacteriol 2016;5, Suppl S1:74-5

How to cite this URL:
Mokaddas E M, Saadaldeen H, Ahmad S. Comparison of two molecular methods and an automated liquid culture system for the early detection of Mycobacterium tuberculosis from both pulmonary and extrapulmonary specimens in Kuwait. Int J Mycobacteriol [serial online] 2016 [cited 2022 Aug 11];5, Suppl S1:74-5. Available from: https://www.ijmyco.org/text.asp?2016/5/5/74/200497




  Conflict of interest Top


None declared.





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