|Year : 2018 | Volume
| Issue : 3 | Page : 212-216
Cervical tuberculous lymphadenitis: Clinical profile and diagnostic modalities
Hitender Gautam, Sonu Kumari Agrawal, Santosh Kumar Verma, Urvashi B Singh
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
|Date of Web Publication||6-Sep-2018|
Dr. Urvashi B Singh
Department of Microbiology, All India Institute of Medical Sciences, New Delhi - 110 029
Source of Support: None, Conflict of Interest: None
Background: Tuberculosis is a major global health problem. Tuberculous lymphadenopathy is a most common form of extrapulmonary tuberculosis (EPTB), constitutes 35% of all cases of EPTB. Due to the paucibacillary nature of specimens, smear microscopy and culture offer low sensitivity. Methods: The aim of the present study was to find the clinicodemographic profiles and comparing the performance of Xpert MTB/RIF, conventional polymerase chain reaction (PCR), mycobacteria growth indicator tube (MGIT) 960, histopathological examination, and clinical follow-up of patients in diagnosing of smear-negative tuberculous lymphadenopathy. Results: A total of 140 clinically suspected cervical tuberculous lymphadenitis cases were enrolled in this study. MGIT-960 culture, conventional PCR, and Xpert MTB/RIF were performed. Most of the patients presented with unilateral (87.14%), single (81.42%), matted (87.85%) lymph nodes, 3 cm–6 cm (52.14%), commonly in the right side (68.02%), and associated lung lesion was found in 12.86% of cases. The detection rates of Mycobacterium tuberculosis complex (MTBC) by Xpert MTB/RIF, conventional PCR, and MGIT were 25.71%, 20.71%, and 17.85%, respectively. Both the tests: Xpert MTB/RIF and PCR, PCR and MGIT, Xpert MTB/RIF and MGIT were positive in 15.71%, 15.71%, and 11.42% of cases, respectively. Most of the patients (74.1%) were cured with 6 months of antitubercular drugs. Conclusion: Clinicians often face the diagnostic dilemmas presented in the study. Individual modalities of the diagnosis are available, but all have drawbacks with varied sensitivity and specificity. Combining the available clinical, radiological, and microbiological modality to reach early diagnosis can go a long way to avoid misdiagnosis and unnecessary delay in treatment, especially in cases, without the pulmonary involvement and fulfilling the aim of National Tuberculosis Control Programme for EPTB cases.
Keywords: Extrapulmonary tuberculosis, lymphadenitis, tuberculosis
|How to cite this article:|
Gautam H, Agrawal SK, Verma SK, Singh UB. Cervical tuberculous lymphadenitis: Clinical profile and diagnostic modalities. Int J Mycobacteriol 2018;7:212-6
|How to cite this URL:|
Gautam H, Agrawal SK, Verma SK, Singh UB. Cervical tuberculous lymphadenitis: Clinical profile and diagnostic modalities. Int J Mycobacteriol [serial online] 2018 [cited 2021 Apr 18];7:212-6. Available from: https://www.ijmyco.org/text.asp?2018/7/3/212/240702
| Introduction|| |
Tuberculosis (TB) is a major public health problem and a significant cause of morbidity and mortality in developing countries., Nearly 9.6 million people get infected, and 1.5 million die from tuberculosis globally every year. Extrapulmonary tuberculosis (EPTB) is seen in nearly 15%–20% of all cases of TB. Tuberculous lymphadenopathy is the most common form of EPTB, constitutes 35% of all cases of EPTB.,,, Cervical lymph nodes are the most common site of tuberculous lymphadenopathy in 60%–90% of cases, and its diagnosis remains a challenge. Due to the paucibacillary nature of specimens, smear microscopy and culture offer low sensitivity., Smear microscopy has been found to be positive in <10% of patients whereas cultures for mycobacteria found to be positive in 39%–80% of cases. Granulomatous lymphadenopathy and caseation-necrosis on histopathological examination may occur in diseases (sarcoidosis, fungal infections, carcinoma, and other inflammatory conditions) other than TB, therefore, may not be very useful., Given the above, relying on a single mode of diagnosis will always have less sensitivity with increased morbidity and mortality. If such an EPTB case has concomitant pulmonary TB, it becomes a source of spread to the community. Thus, the use of conventional methods and histopathological examination in conjunction with molecular techniques is all to be done in conjunction to cover-up the low-sensitivity issue of individual tests and further help in the detection of Mycobacterium tuberculosis (MTB) in tuberculous lymphadenitis.
The aim of the present study was to find the clinicodemographic profiles and comparing the performance of Xpert MTB/RIF, conventional polymerase chain reaction (PCR), mycobacteria growth indicator tube (MGIT) 960, histopathological examination, and clinical follow-up of patients in the diagnosis of smear-negative tuberculous lymphadenopathy.
| Methods|| |
This study was conducted at the Tuberculosis division, Department of Microbiology, All India Institute of Medical Sciences, New Delhi, between May 2015 and May 2016. The age of the patients ranged from 9 to 85 years of age. The patients with cervical lymphadenopathy were investigated for tubercular etiology. A total of 140 patients with enlargement of cervical lymph node or swelling of the neck or draining sinus presenting to different specialties of our hospital were studied in detail. As per the Revised National Tuberculosis Control Programme (RNTCP), fine-needle aspiration (FNA) was performed in all the patients after detailed clinical history and examination. Excision biopsy was done in case of negative and doubtful FNA cytology (FNAC) results. Erythrocyte sedimentation rate (ESR), tuberculin skin test, and chest radiograph were done in all the patients.
Collection and processing of samples
FNA/excision biopsy from 140 patients was received in TB division (Department of Microbiology, All India Institute of Medical Sciences, New Delhi) for smear examination Ziehl–Neelsen (ZN) staining, culture (MGIT 960 system), Xpert MTB/RIF, and PCR. Another part was subjected to histopathological examination. The samples were processed using the NALC-NaOH method (N-acetyl-L-cysteine-sodium citrate method) as per standard method for making smear, culture, Xpert MTB/RIF, and PCR tests.
After processing, the samples were subjected to smear examination using ZN staining method and examined under light microscope, and interpretation was done as per RNTCP guidelines.
Mycobacteria growth indicator tube 960
Decontaminated samples were inoculated into MGIT tube (containing 7H9 medium), MGIT 960 nonradiometric automated isolation system (Becton Dickinson, Sparks, MD, USA), and positive cultures were further confirmed using TBc identification test (TBcID, Becton Dickinson, Sparks, MD, USA).
The Xpert was performed as per manufacturer's instructions (Cepheid, Sunnyvale, CA). In this assay, sample reagent was added into clinical samples at a 2:1 ratio and was incubated for 10 min. At room temperature, shaken again and kept for 5 min, then 3 ml of inactivated material was transferred to the cartridge. The cartridges were inserted into test platform and results were produced after 90 min.,, The interpretation of data from tests was software based and not user dependent.
DNA isolation and polymerase chain reaction
Sample and reagent preparation and PCR amplification were carried out in separate rooms using utmost precautions to minimize crossover and carryover contamination. DNA extraction was standardized by phenol chloroform isoamyl alcohol method using the lysis buffer containing 20 mM Tris HCL, 0.5% Tween 20, and 1 mg/ml proteinase k for 16 h at 56°C. The DNA was amplified using primer sequences MPT1 (5'-TCC GCT GCC AGTCGT CTT CC-3') and MPT2 (5'-GTC CTC GCG AGTCTA GGC CA-3') in 50-μl reaction mixtures as per previously published protocol. These primers generate amplicons of 240 bp for MPT-64 gene for MTB. During each PCR, appropriate positive control (100 pg of H37Ra DNA) and negative controls (sterile distilled water) were run with the samples. The amplified products were analyzed by gel electrophoresis.
Composite reference standards (CRS) have been considered as gold standard in the present study to overcome such issues. CRS for aspirates and biopsy samples include the following: any two of culture/histopathology/radiological findings/response to treatment positive. Response to treatment was assessed regarding the improvement of signs and symptoms such as fever, weight gain, improvement in general well-being, and decrease in size of the lymph node.
Data were collected and analyzed using STATA/SE 14.0 statistical software (Stata Corp LLC, 4905 Lakeway Drive college station, Texas, USA). Categorical data were described using numbers and percentages. Our data were presented in the form of tables, and descriptive statistics were analyzed in percentages. P value was calculated using Chi-square test to analyze statistical significances.
| Results|| |
Clinical and demographic characteristics of study population
A total of 140 patients with cervical lymphadenopathy were included in the study. Among these 140 patients, 65 (46.42%) were male, and 75 (53.57%) were female; the male-to-female ratio was 1:1.15. The age of the patients ranged from 9 to 85 years with a mean age of 59.55 ± 14.46 years. Among these 140 patients, 123 (87.85%) patients presented with solid lymph node, 14 (10%) with abscess, and 3 (2.14%) with discharging sinus. Unilateral cervical lymph node involvement was seen in 122 (87.14%) cases, more in the right side 83 (68.02%) than 39 (31.96%) left side. Lymph node size of 3cm–6 cm in diameter was seen in 74 (52.85%) cases, followed by >6-cm diameter in 42 (30%), and <3-cm diameter in 24 (17.14%) cases, and associated lung lesion was found in 18 (12.86%) by X-ray cases. There was no significant relationship between site of involvement and enlarged lymph node with gender of the patients. The systemic features, such as fever 105 (75%), weight loss 83 (59.28%), and night sweats 81 (57.85%), were found in patients [Table 1]. ESR was raised in 67 (47.85%) patients, and the Mantoux test was positive in 44 (31.42%) cases. All samples were negative by smear microscopy.
Performance of Xpert MTB/RIF, polymerase chain reaction, mycobacteria growth indicator tube 960, and fine-needle aspiration cytology for diagnosis of cervical tuberculous lymphadenitis
Among the 140 suspected cervical tubercular lymphadenitis patients, detection rates of Mycobacterium tuberculosis complex (MTBC) by Xpert MTB/RIF, conventional PCR, and MGIT 960 were 25.71%, 20.71%, and 17.85%, respectively. Xpert MTB/RIF detected16 cases, which were negative by MGIT960 while in five cases were missed by Xpert MTB/RIF which were positive by MGIT960. There were eight culture-positive cases, which were negative on PCR and 12 culture-negative cases were PCR positive. Xpert MTB/RIF and PCR, PCR and MGIT 960, and Xpert MTB/RIF and MGIT 960 were positive in 15.71%, 15.71%, and 11.42% cases, respectively [Table 2]. All microbiological tests (MGIT 960, Xpert MTB/RIF, and PCR) were positive in 11.42% of cases. Sensitivity and specificity of GeneXpert and PCR against gold standard are shown in [Table 3].
|Table 2: Comparative analysis of composite reference standard, Xpert MTB/RIF, conventional polymerase chain reaction, and mycobacteria growth indicator tube for the diagnosis of cervical tubercular lymphadenitis|
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|Table 3: Sensitivity and specificity of GeneXpert and polymerase chain reaction against gold standard as shown in table|
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Response to treatment
Category I treatment was started in 80 (57.14%) patients as per RNTCP guidelines and most of these patients (75%) responded within 6 months of treatment, 13.75% of patients cured within 9 months, and 11.25% needed 1 year of treatment. All the Xpert MTB/RIF positive cases were rifampicin sensitive.
There was no reduction in the size of the lymph node in six (4.29%) cases even after antitubercular treatment; however, there was an improvement in general well-being and weight gain. In these six patients, finally, surgery was done to remove the lymph nodes. About 7.85% (n = 11) of cases were negative by all the three microbiological (MGIT 960, Xpert MTB/RIF, and PCR) investigations as well as inconclusive FNAC results. The antitubercular treatment was started in these patients on the basis of clinical suspicion such as raised ESR, positive Mantoux test, and the size of the lymph node decreased in these 11 cases also within 6 months of the treatment.
| Discussion|| |
TB is a major cause of morbidity and mortality worldwide. In India, tuberculous lymphadenopathy is one of the most common types of lymphadenopathy encountered. Both tuberculous and nontuberculous mycobacteria can be responsible for cervical lymphadenitis and its diagnosis, and management is still a problem despite its increasing worldwide incidence.,, TB is considered as the most common opportunistic infection in belts where HIV infection is rampant.
Male:female ratio (1:1.5) of the patients in this study was quite consistent with the studies in Pakistan and India; however, differences were found with the studies done in UK and Pakistan.,,, Jha et al. found the number of patients having a cervical abscess or sinus quite low which was consistent with this study. In our study, abscess was seen in 10% of cases and discharging sinus in 2.14% of cases while it was found in higher percentage in a study done in Bangladesh in which abscess was seen in 21.5% and sinus formation in 9.2% of cases. Our study found unilateral neck swelling (87.14%) as the most common presentation which was similar to other studies., On chest radiograph, in our study, associated lung lesion was found in 12.86% of cases; whereas in other studies, it was seen in 16% and 7.5% of cases; while Choudhury et al. found associated lung lesions in 48.48% of cases.,, In our study, ESR was found raised in 47.85% of cases whereas Magsi et al. and Umer et al. found ESR raised in 12.5% and 47.7% of cases, respectively.,
The diagnosis of EPTB is challenging for a number of reasons: the paucibacillary nature of the specimens, the lack of adequate sample amounts, or volumes, the apportioning of the sample for various diagnostic tests (histology/cytology, biochemical analysis, microbiology, and PCR), resulting in a nonuniform distribution of microorganisms.
Taking this into account, the present study was done to study the clinical profile and diagnostic modalities for diagnosing tuberculous lymphadenopathy. Among these 140 suspected cervical tubercular lymphadenitis patients, detection rates of MTBC by Xpert MTB/RIF, conventional PCR, and MGIT 960 were 25.71%, 20.71%, and 17.85%, respectively. Xpert MTB/RIF detected 16 cases which were negative by MGIT 960. There were five culture-positive cases, which were negative on Xpert MTB/RIF, this may be due to inhibition in the FNA sample. Although histopathology is an inexpensive and reliable tool for detecting tubercular lymphadenitis cases in resource-limited settings, studies have highlighted the limitations of associating specific histopathological features with TB. Nevertheless, histopathology can be invaluable in arriving at specific tissue diagnosis in diseases clinically mimicking TB such as lymphomas. Although the conventional methods (smear microscopy/culture) were used as a reference standard, these methods are not sufficient to detect all tubercular lymphadenitis cases as shown in our study. If we had gone by a single test, we would have missed true TB cases in which antitubercular therapy was started on the clinical ground and improved.
Given treatment response in 11 cases, which were negative by all the microbiological investigations as well as inconclusive FNAC results, response to treatment adds to the clinical diagnosis. These limitations of all individual tests point toward the significance of CRS [Table 2]. Antituberculous chemotherapy is the mainstay in the management of TB lymphadenitis. The 6 months' treatment may be sufficient for many patients (75%); however, it is difficult to define a clear-cut “end point” for assessing the efficacy of treatment of EPTB with delayed response. Six patients finally needed some form of surgical interventions to remove the lymph nodes. In a study by Jha et al., most of the patients treated successfully with short-course chemotherapy for 6 months while surgery was required sometimes.
| Conclusion|| |
Clinicians often face the diagnostic dilemmas presented in the study. Individual modalities of diagnosis are available but all have drawbacks with varied sensitivity and specificity. Combining the available clinical, radiological, and microbiological modality to reach early diagnosis can go a long way to avoid misdiagnosis and unnecessary delay in treatment, especially in cases, without pulmonary involvement and fulfilling the aim of national tuberculosis control program for EPTB cases. In the current study, CRS criteria were better than conventional method/s for the diagnosis of cervical lymphadenitis cases, and early initiation of antitubercular therapy reduces the transmission of TB in community due to high chances of concurrent pulmonary tuberculosis.
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Conflicts of interest
There are no conflicts of interest.
| References|| |
Chakravorty S, Sen MK, Tyagi JS. Diagnosis of extrapulmonary tuberculosis by smear, culture, and PCR using universal sample processing technology. J Clin Microbiol 2005;43:4357-62.
World Health Organization. Global Tuberculosis Control: Surveillance, Planning, Financing. W.H.O./CDS/TB/2002.295. Geneva, Switzerland: World Health Organization. Available from:apps.who.int/iris/bitstream/10665/63835/13/WHO_CDS_TB_2002.295_eng.pdf. [Last accessed on 2018 Aug 21].
Das S, Das D, Bhuyan UT, Saikia N. Head and neck tuberculosis: Scenario in a tertiary care hospital of North Eastern India. J Clin Diagn Res 2016;10:MC04-7.
Hegde S, Rithesh KB, Baroudi K, Umar D. Tuberculous lymphadenitis: Early diagnosis and intervention. J Int Oral Health 2014;6:96-8.
Appling D, Miller RH. Mycobacterium cervical lymphadenopathy: 1981 update. Laryngoscope 1981;91:1259-66.
Hooper AA. Tuberculous peripheral lymphadenitis. Br J Surg 1972;59:353-9.
Krishnaswami H, Koshi G, Kulkarni KG, Job CK. Tuberculous lymphadenitis in South India-a histopathological and bacteriological study. Tubercle Lung Dis 1972;53:215-20.
Prakash UB, Reiman HM. Comparison of needle biopsy with cytologic analysis for the evaluation of pleural effusion: Analysis of 414 cases. Mayo Clin Proc 1985;60:158-64.
Singh UB, Bhanu NV, Suresh VN, Arora J, Rana T, Seth P, et al.
Utility of polymerase chain reaction in diagnosis of tuberculosis from samples of bone marrow aspirate. Am J Trop Med Hyg 2006;75:960-3.
Kubica GP, Dye WE, Cohn ML, Middlebrook G. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am Rev Respir Dis 1963;87:775-9.
Central TB Division Ministry of Health and Family Welfare. Training Manual for Mycobacterium tuberculosis
Culture & Drug Susceptibility Testing. New Delhi, India: Central TB Division Ministry of Health and Family Welfare; 2009.
Siddiqi SH, RuschGerdes S. MGIT Procedure Manual. Geneva, Switzerland: Foundation for Innovative New Diagnostics; 2006.
Boehme CC, Nabeta P, Hillemann D, Nicol MP, Shenai S, Krapp F, et al.
Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med 2010;363:1005-15.
Blakemore R, Story E, Helb D, Kop J, Banada P, Owens MR, et al.
Evaluation of the analytical performance of the xpert MTB/RIF assay. J Clin Microbiol 2010;48:2495-501.
World Health Organization. Standard Operating Procedure [SOP]: Specimen processing of CSF, lymphnodes and other tissues for Xpert MTB/RIF. World Health Organization; 2014.
Singh UB, Pandey P, Mehta G, Bhatnagar AK, Mohan A, Goyal V, et al.
Genotypic, phenotypic and clinical validation of geneXpert in extra-pulmonary and pulmonary tuberculosis in India. PLoS One 2016;11:e0149258.
Chand P, Dogra R, Chauhan N, Gupta R, Khare P. Cytopathological pattern of tubercular lymphadenopathy on FNAC: Analysis of 550 consecutive cases. J Clin Diagn Res 2014;8:FC16-9.
Tortoli E. Epidemiology of cervico-facial pediatric lymphadenitis as a result of nontuberculous mycobacteria. Int J Mycobacteriol 2012;1:165-9. [Full text]
Smaoui S, Mezghanni MA, Hammami B, Zalila N, Marouane C, Kammoun S, et al.
Tuberculosis lymphadenitis in a Southeastern region in Tunisia: Epidemiology, clinical features, diagnosis and treatment. Int J Mycobacteriol 2015;4:196-201. [Full text]
Reuss A, Drzymala S, Hauer B, von Kries R, Haas W. Treatment outcome in children with nontuberculous mycobacterial lymphadenitis: A retrospective follow-up study. Int J Mycobacteriol 2017;6:76-82.
] [Full text]
Nanda KD, Mehta A, Marwaha M, Kalra M, Nanda J. A disguised tuberculosis in oral buccal mucosa. Dent Res J (Isfahan) 2011;8:154-9.
Ahmed I, Hashmi S, Tanwir F, Ahmed S, Khan MS. Tuberculosis – Frequency and differential diagnosis – analysis of cases in Pakistan. Oral Health Dent Manag 2014;13:768-71.
Jha BC, Dass A, Nagarkar NM, Gupta R, Singhal S. Cervical tuberculous lymphadenopathy: Changing clinical pattern and concepts in management. Postgrad Med J 2001;77:185-7.
Magsi PB, Jamro B, Shaikh AA, Sangi HA. An audit of 140 cases of cervical lymphadenopathy at tertiary care hospital. Golam J Med Sci 2013;11:47-9.
Choudhury N, Bruch G, Kothari P, Rao G, Simo R. 4 years' experience of head and neck tuberculosis in a South London hospital. J R Soc Med 2005;98:267-9.
Kamal MS, Hoque MH, Chowdhury FR, Farzana R. Cervical tuberculous lymphadenitis: Clinico-demographic profiles of patients in a secondary level hospital of Bangladesh. Pak J Med Sci 2016;32:608-12.
Fontanilla JM, Barnes A, von Reyn CF. Current diagnosis and management of peripheral tuberculous lymphadenitis. Clin Infect Dis 2011;53:555-62.
Umer MF, Mehdi SH, Muttaqi AE, Hussain SA. Presentation and aetiological aspect of cervical lymphadenopathy at Jinnah medical college hospital Korangi, Karachi. Pak J Surg 2009;25:224-6.
[Table 1], [Table 2], [Table 3]
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