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ORIGINAL ARTICLE
Year : 2021  |  Volume : 10  |  Issue : 1  |  Page : 43-50

The biofilm formation of nontuberculous mycobacteria and its inhibition by essential oils


1 Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center Ural Branch of RAS; Faculty of Soil Science, Agrochemistry, Ecology and Merchandising, Perm State Agro-Technological University, Perm, Russia
2 Institute of Ecology and Genetics of Microorganisms; Institute of Technical Chemistry, Perm Federal Research Center Ural Branch of RAS, Perm, Russia
3 Faculty of Soil Science, Agrochemistry, Ecology and Merchandising, Perm State Agro-Technological University, Perm, Russia

Correspondence Address:
Daria V Eroshenko
Perm Federal Research Center Ural Branch of RAS, Institute of Ecology and Genetics of Microorganisms, Goleva 13, Perm 614010
Russia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijmy.ijmy_228_20

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Background: Nontuberculous mycobacteria (NTM) form two types of biofilms: Bottom biofilm and pellicle. The spatial distribution of cells between these types of biofilms and their dispersion into the liquid medium depends on the ratio of the nutrient components of the growth medium. The inhibition of biofilm formation by NTM can be achieved through the use of lipophilic compounds, such as essential oils (EOs). Method: The biofilm and pellicle formation of Mycobacterium smegmatis and Mycobacterium avium on four nutrient media under static conditions and in the vapors of six EOs was evaluated by conventional method. The antimycobacterial effect of EOs was also studied by the disc diffusion method. Results: The bottom biofilm and pellicle formation of NTM largely depended on the composition and availability of nutrients. Nutrient media in which NTM form powerful bottom biofilm or pellicle or both have been determined. The growth of studied NTM strains on agar was highly sensitive to the EOs of Scots pine, Atlas cedar, bergamot, and a mixture of EO of different plants. The cultivation of bacteria in the EO vapors also resulted in total suppression of the pellicle for all studied NTM strains. Conclusions: Our data clearly indicate that the carbon-nitrogen ratio is involved in the regulation of the spatial distribution of the biofilm. The preventing effect of EOs vapors, especially the synergistic action of mixture of EOs on the biofilm and pellicle formation by NTMs can be observed.


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